FastQCFastQC Report
Tue 22 May 2018
HVKKNBGX5_n01_a10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKKNBGX5_n01_a10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6654014
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG25000.014.7666669
GGCAGAA104500.014.06474369
CTATACT14550.013.516884
GTATTAT33450.013.4102831
GTATAGA10900.013.2957711
GACAGGC231050.013.21526269
TATACAG15850.013.0614035
AGGCAGA133250.013.04973969
CAGGCAG156450.013.0331869
TATAGTG12000.012.9389525
ACAGGCA199100.012.84060369
GTATAAT19850.012.689751
CCTAGGC5850.012.3859133
GTCTTAG11350.012.1605961
GCAGAAA91900.012.08867969
TATACCG6600.012.0240775
GTATAGT17000.011.9755041
GTATATA16000.011.8613321
GTATTAA33250.011.8305221
TTATACT18150.011.4061544
Produced by FastQC (version 0.11.2)