FastQCFastQC Report
Sat 4 May 2024
HVKGJDMXY_n01_HT291_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKGJDMXY_n01_HT291_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5759247
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGGGATATCTCGTAT254290.44153341573993965TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGGGATATCGCGTAT114600.19898434639111676TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTG77280.134184208456418TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)