FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32524235
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG412200.12673626297436358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA13400.026.77634459
AGCGATC679300.020.17331369
CGTATCA18600.020.03291962
GCGATCT478900.019.34089969
CCGTATC20800.017.74790861
TCGCCGT20950.017.12697858
GCCGTAT21800.016.77531860
TAGGACG208900.016.2157234
CGATCTA335750.016.11643669
GAGCGAT891900.015.92955769
GTCCTAC223600.015.18611
GTAGGAC221000.014.8603373
AGGACGT227500.014.6924085
GGACGTG224000.014.2446676
CGTGATT102300.014.06303828
CCGTGAT103550.013.89316927
GATCTAG314550.013.59316569
TATGGCG203150.013.39654316
CGAGCGA1101400.013.1591768
GACGAGC1650050.012.84342869
Produced by FastQC (version 0.11.2)