FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27588573
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCT442250.018.99256569
AGCGATC610200.018.75310769
CGCCGTA11650.017.17618459
CGATCTA312800.016.60353969
TAGGACG106950.015.4815124
GAGCGAT782550.014.94434968
CGTATCA14050.014.24269162
GTCCTAC131500.014.2228151
GTAGGAC127400.013.5926393
AGGACGT120600.013.3857295
GATCTAG281150.013.07201169
GACGAGC1426050.012.75283569
AGCGATA18300.012.634469
GGACGTG130400.012.1683266
CGAGCGA970250.012.1528768
CCGTATC17450.012.06047861
GCCGTAT16350.012.0276160
TATGGCG107650.011.95169816
ACGAGCG1143900.011.75033769
GTATACG10150.011.5591231
Produced by FastQC (version 0.11.2)