FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31651510
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG46200.031.147511
TTGACGG56950.024.11003131
CTACGGT35650.023.70564813
TACGGTG36400.023.40644314
CGCCGTA9200.022.1254759
GCGATCT249300.021.64891869
AGCGATC364000.021.51071469
TGACGGC64900.021.3689732
TCCGCAA40500.021.12360633
CTAGACG42200.020.9270743
GACGGCT65700.020.68722533
CGACGAC17000.019.89293369
TAGACGC73150.019.5703984
CCTACGG44200.019.35451512
CGTATCA10750.019.25695262
GACGCAC45650.018.9670686
ACGCACC45750.018.925557
CGGCTAT78250.018.4270235
GTCCGCA47450.018.10361932
TACGCCG19300.018.0520215
Produced by FastQC (version 0.11.2)