FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36973727
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA25750.051.18063759
TCGCCGT29700.044.25936558
CGTATCA30850.043.05701462
CCGTATC33700.039.31204261
GTGGTCG39700.033.20414754
GTCGCCG40750.032.51455757
GATCTCG48300.027.64677847
GCCGTAT48000.027.38407360
CGTCTAG37000.026.7677941
TCTCGGT49650.026.41004449
TCGGTGG49450.026.23933251
CGACGAC19500.026.19148369
ATCTCGG50950.025.93848848
TGGTCGC54350.025.1424455
CGGTGGT52950.024.56994852
GGTCGCC59650.022.27169656
CTCGGTG62000.021.31725550
AGCGATC716050.021.19555969
TTGACGG44250.020.66031331
CTACGGT28950.020.2557213
Produced by FastQC (version 0.11.2)