FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31991837
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG441990.13815711801732422No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAA330950.10344826400559617Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA42900.057.74180659
TCGCCGT48600.051.04140558
CGTATCA49100.051.01469462
CCGTATC49650.050.3096561
GTGGTCG56850.043.9471254
GTCGCCG57300.043.59769457
GCCGTAT59250.042.27394560
TGGTCGC61200.040.59718355
TCTCGGT69100.036.90420549
TCGGTGG68850.036.68943451
GATCTCG69150.036.27682547
ATCTCGG71150.035.50068748
CGGTGGT72350.034.9143352
GGTCGCC78200.032.03567556
CTCGGTG84550.030.16170350
GTAGATC111800.022.7749544
CGTCTAG29550.021.837851
AGCGATC726750.019.44026269
GTATCAT131950.019.29724563
GGTGGTC142400.017.93274753
Produced by FastQC (version 0.11.2)