FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33349275
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1805440.5413730883205108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA24950.050.6094659
CGTATCA29450.042.87806362
TCGCCGT30750.041.17716258
CCGTATC31200.040.9144561
GTGGTCG38750.032.94930654
GTCGCCG40300.031.50817157
GATCTCG43750.029.41839647
CGTCTAG38400.029.026071
ATCTCGG44950.028.86328348
TCGGTGG46250.028.12900451
TCTCGGT46850.027.69364749
TGGTCGC48400.026.73591255
GCCGTAT48200.026.26867960
CGGTGGT50500.025.48822452
CTCGGTG56100.022.94370550
TTGACGG47900.021.9667931
AGCGATC435000.020.06258669
TGACGGC53050.019.96417432
CTACGGT32300.019.00882513
GCGATCT309450.018.86849469
Produced by FastQC (version 0.11.2)