FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30891021
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA15900.045.7828459
CGTATCA19950.036.31764662
TCGCCGT20750.035.249258
CCGTATC22300.032.6444861
GTGGTCG27800.026.56403454
GTCGCCG28950.025.74462757
GCCGTAT30300.024.36620360
CGTCTAG25000.022.7761081
TGGTCGC34350.021.69919455
GATCTCG34550.021.17266747
AGCGATC645550.021.01941569
GCGATCT449150.019.89964769
ATCTCGG37350.019.67825548
TCGGTGG37600.019.64084251
CGGTGGT39200.018.7510852
TCTCGGT38950.018.69320949
GGTCGCC40500.018.3185156
CTACGGT24200.017.24658213
TAGGACG177850.016.8937824
GGTACGA22350.016.35904118
Produced by FastQC (version 0.11.2)