FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34766609
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG469410.13501748186025275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA12000.029.61257659
CGTCTAG33150.023.8381611
AGCGATC786600.021.9484469
CGTATCA17400.021.21645762
GCGATCT534700.020.5747869
TTGACGG36450.019.59205231
TCGCCGT19300.018.77007158
CCGTATC20250.018.40044461
GAGCGAT1040150.017.0022768
CGGCTAT49200.016.75675435
CTACGGT27650.016.7170613
TAGGACG189650.016.4612274
TCCGCAA28100.016.20481733
TGACGGC44350.016.10205732
CGATCTA366500.015.90313569
TAGACGC49300.015.81354
CGTCTAA27800.015.7644831
GACGGCT44650.015.60653233
GGACGTG193550.015.3808076
CCGACGC47800.015.377254569
Produced by FastQC (version 0.11.2)