FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29228748
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360970.12349827642292445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA18900.048.55684759
CGTATCA20600.044.3834762
TCGCCGT22450.040.87915458
CCGTATC22750.040.3400261
GTGGTCG28600.032.21463454
CGTCTAG32550.031.379641
GATCTCG32200.028.82540547
GTCGCCG32650.028.42792157
TCTCGGT33650.027.27624149
ATCTCGG34500.026.80360248
TCGGTGG34900.026.79529251
CGGTGGT35700.026.00138752
CTACGGT28800.022.87662913
TACGGTG28800.022.7566614
GCCGTAT40800.022.57746160
TGGTCGC42000.022.4293655
GGTCGCC41600.022.2299456
TTGACGG44550.021.60552831
AGCGATC560300.021.5685769
CTCGGTG45500.020.62814950
Produced by FastQC (version 0.11.2)