FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39683014
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA36900.049.36617359
CGTATCA41200.044.6343162
TCGCCGT46150.039.69706358
CCGTATC45950.039.49402661
GCCGTAT51300.035.44143760
GTGGTCG59300.031.71430454
TGGTCGC62550.029.7902655
GATCTCG68450.027.57389847
ATCTCGG71350.026.11500548
GTCGCCG76150.024.6491157
TCTCGGT76850.024.51617849
TCGGTGG77600.024.23592651
CGGTGGT81300.023.38740552
CGTCTAG36850.019.6653021
CTCGGTG97700.019.49687250
AGCGATC696550.018.37550269
GGTCGCC103150.018.2316256
GCGATCT510850.017.88789669
GTAGATC115300.016.63773544
TTGACGG40250.016.11361931
Produced by FastQC (version 0.11.2)