FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36243343
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG506200.13966702795600283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA27850.049.67596459
CGTATCA32250.043.11385362
TCGCCGT35150.039.55669858
CCGTATC36500.038.66036661
GTGGTCG44250.032.0517454
GCCGTAT47750.029.18982560
GTCGCCG50150.028.07207557
TGGTCGC51000.027.87675955
GATCTCG52000.027.53788647
ATCTCGG54900.026.2094948
CGTCTAG35400.025.4425641
TCTCGGT58700.024.51319949
TCGGTGG59200.024.13309951
CGGTGGT61000.023.36417452
AGCGATC494600.019.72461569
TTGACGG43100.019.45056231
CTCGGTG76850.019.26350250
GCGATCT354350.017.81222269
GGTCGCC79850.017.6748856
CGTACGT26250.017.47730834
Produced by FastQC (version 0.11.2)