FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n02_ba87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n02_ba87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40205699
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG501250.12467138054234549No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA27100.048.37779259
CGTATCA27500.047.8009462
CCGTATC30500.043.32453561
TCGCCGT33500.038.92992458
CGTCTAG74850.038.448511
GTGGTCG40200.033.13347654
GATCTCG42000.032.36865247
ATCTCGG44450.030.73988248
TTGACGG94400.029.56544931
TCTCGGT48250.028.17673349
TGACGGC103500.027.09910232
GACGGCT104000.026.53576733
GTCGCCG49650.026.40803357
TCGGTGG51100.026.33633851
CGGTGGT51400.025.91402252
CTACGGT48750.024.26953913
TACGGTG50550.023.54181714
TGGTCGC58300.023.02406155
CGGCTAT132200.021.94443535
CTCGGTG64200.021.82232950
Produced by FastQC (version 0.11.2)