FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32524235
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGGC818600.017.26655269
ATCTCGT58750.017.26437669
TAGGACG222400.016.2572064
GTAGGAC228400.015.8301583
GATCTCG76300.015.78019169
CGTGATT102850.015.76644228
CCGTGAT103050.015.50178727
CTAGGCA626750.015.40706669
GTCCTAC219600.015.2877411
AGGACGT240950.014.9053875
GGACGTG237050.014.5684356
GACTAGG1015100.014.48491869
TTTTCCG118000.013.62561223
TCCGTGA116300.013.46880126
TTTCCGT118050.013.35683824
TATGGCG213700.013.33460816
TGTAGGA296550.013.3325362
GACGTGG135800.013.2864417
TTCCGTG120450.013.23390325
TAGGCAT434750.012.72053469
Produced by FastQC (version 0.11.2)