FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27588573
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT55050.022.18468769
ACGGACT613750.018.66743769
TATCTCG66350.018.66644968
CGGACTC461950.018.35668469
TAGGACG113950.015.0778314
GTAGGAC137200.014.5343643
GACGGAC813750.014.52034469
GTCCTAC129300.014.0360521
AGGACGT127300.013.4153335
CGAGACG1439200.012.69504369
GGACGTG134050.012.2508336
TGTAGGA186500.012.2464032
AGACGGA976800.012.19548568
GGACTCC431350.012.18084669
ACGAGAC1726750.011.51001169
CGTGATT58600.011.48089728
GAGACGG1206000.011.40814969
TACACTG165050.011.3503425
CTATACT87350.011.3355574
TTGACGA69200.011.267260
Produced by FastQC (version 0.11.2)