FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31651510
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG44550.032.993171
TTGACGG55100.025.3592731
CTACGGT39500.022.62104413
TACGGTG39800.022.36367614
CTAGACG42750.022.2736743
TGACGGC62850.022.17719832
GACGGCT64250.021.26454433
GACGCAC45300.020.4867786
ACGCACC44600.020.1891357
TCCGCAA43600.019.7036633
CCTACGG45800.019.58483512
TAGACGC76100.019.2674054
CGGCTAT76900.019.06753535
ACGGTGA48850.018.57361215
CGTACGT34650.018.12163434
CCGTACG35450.017.71287733
GTCCGCA49050.017.65490232
CGTCTTA30550.017.61645715
GGTACGA44050.017.23046718
TTATCGT38000.016.97703450
Produced by FastQC (version 0.11.2)