FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36973727
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG34400.028.9863821
TATGCCG59000.020.46544849
ACGTAGA761900.020.15894169
TTGACGG44700.020.14485231
CTACGGT31950.019.54405813
CCGTCTT56850.019.23685653
TCCGCAA32200.019.17915333
TGCCGTC59450.019.09193251
GGTACGA34200.019.06595618
GCCGTCT59200.018.53150652
TACGGTG34300.018.40616814
TGACGGC50100.018.38666232
TAGACGC68700.018.0785394
CGTAGAG541800.017.95645769
GACGCAC37050.017.7854376
CCGTACG30300.017.76291733
CTCGTAT59650.017.69772345
CGTACGT30450.017.67529334
ATGCCGT67100.017.48081450
GTACGAG37650.017.31913419
Produced by FastQC (version 0.11.2)