FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31991837
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC85600.029.21904251
CCGTCTT86000.028.72215553
GCCGTCT86800.028.53687952
TATGCCG89450.028.2314549
ATGCCGT91300.027.69718650
CGTCTAG29550.027.2057861
CTCGTAT87600.025.75586145
TCTCGTA90700.024.60946344
CGTCTTC112050.021.98312654
ATCTCGT106250.020.71560343
TTGACGG36750.020.18425631
TCGTATG127950.020.14107946
CGTATGC136400.018.58982847
GGTACGA22300.018.56532718
GTATGCC138800.018.54179648
TGACGGC41000.018.26022732
CGGCTAT43750.018.0589235
TAGGACG145850.017.6226524
TAGACGC47650.017.0871854
TCCGCAA25650.016.81348233
Produced by FastQC (version 0.11.2)