FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33349275
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG37750.028.9740891
TATGCCG46700.027.62873349
TGCCGTC48050.027.35506851
GCCGTCT49700.026.30799552
CCGTCTT49650.025.91758553
CTCGTAT49350.024.39745145
TCTCGTA50550.022.72680744
TTGACGG46000.022.6504531
ATGCCGT58050.022.6427450
CTACGGT35200.020.28784213
ATCTCGT56200.020.01225343
TGACGGC53050.019.77039132
ACCGAGG469100.019.2392369
TACGGTG37800.018.9835114
GACGGCT55500.018.5869133
CTAGACG41100.018.0475883
CCGTACG29550.017.86335233
TCCGCAA39350.017.79837433
TAGACGC70900.017.7123554
GACGCAC41350.017.5212466
Produced by FastQC (version 0.11.2)