FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30891021
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG25400.026.7608411
ATCTCGT54000.022.42476369
ACGCTAC674150.020.94081769
TGCCGTC31050.020.33262651
CGCTACG453000.020.15144269
TATGCCG33400.019.83167649
TTGACGG31250.019.54148131
TATCTCG71000.019.1934669
CTCGTAT34400.018.75370845
TCTCGTA35400.018.41923744
CCGTCTT33650.018.35151553
GCCGTCT34100.018.31167852
ATGCCGT35950.018.23305150
CTACGGT23350.017.28622413
TAGGACG184000.017.0813474
GGTACGA22050.016.8978918
CTACGCG13350.016.79757569
GTCCTAC214550.016.2106061
TGACGGC37700.016.19807232
TAGACGC45150.015.970194
Produced by FastQC (version 0.11.2)