FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34766609
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32750.023.599061
CTACGGT31500.018.61830713
GATCGCG24600.018.5118369
TTGACGG39150.017.97761331
TCCGCAA31700.017.95814333
ACCAGAG1025700.017.61796269
TAGACGC54500.017.5349454
TACGGTG33300.017.40476214
CTAGACG35500.017.3958323
GACGCAC37850.016.4981926
ATCTCGT31650.016.24138569
TAGGACG192550.016.179514
TTATCGT29350.015.86825950
GTCCGCA38000.015.61628932
GGTACGA25000.015.59423118
GATCTCG47600.015.4377269
CGGCTAT50350.015.21207335
TGACGGC48650.015.03439732
GACCAGA1202050.015.02750968
CGTGATT94650.015.01819528
Produced by FastQC (version 0.11.2)