FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29228748
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29650.031.06641249
CGTCTAG34750.030.4820771
CCGTCTT30850.029.07509853
TGCCGTC31000.029.04580151
GCCGTCT34750.026.30833652
CTCGTAT35350.025.76434145
ATCTCGT50500.024.52517169
CTACGGT31350.024.20967113
TCTCGTA37600.023.94774444
TACGGTG31850.023.72125814
TTGACGG42750.023.56581731
CTAGACG37450.021.3721833
TCCGCAA35900.021.14290633
GACGCAC36800.020.9062296
CCTACGG36150.020.80445912
TGACGGC49400.020.53310432
ATGCCGT45850.020.16508350
ACGGTGA38800.020.00566915
ACGCACC38600.019.8416087
GACGGCT50800.019.28817433
Produced by FastQC (version 0.11.2)