FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39683014
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG74150.024.42606549
ATGCCGT78550.023.05783350
CTCGTAT75150.022.26476745
TGCCGTC82450.022.2600951
CGTCTAG35750.022.101251
CCGTCTT80650.021.98687753
GCCGTCT83650.021.44579952
TCTCGTA78150.021.41031644
ATCTCGT92000.017.62464343
TCGTATG114550.016.74497646
TTGACGG42700.016.48293131
CGTCTTC108300.016.37344454
CGGCTAT48900.015.09846235
CGTATGC122800.015.0019747
GTATGCC128100.014.89297248
TAGACGC53400.014.6657854
GGTACGA28900.014.56408618
TAGGACG183500.014.5144954
CTACGGT31600.014.08336313
CTAGACG38050.013.6005253
Produced by FastQC (version 0.11.2)