FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36243343
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG34050.025.3328381
CTCGTAT54750.024.63753545
TGCCGTC59250.024.2219851
TATGCCG59200.024.12588549
CCGTCTT59050.023.71985253
TCTCGTA58600.023.01917544
ATGCCGT63700.022.63817850
GCCGTCT64600.022.3761952
ACCGTAC497900.019.63685469
TTGACGG43300.018.72437531
ATCTCGT71850.018.29395543
CTACGGT31900.016.5464713
TAGACGC57400.016.3485414
CTAGACG35050.016.3396683
TCCGCAA32150.016.3114933
TGACGGC50550.016.24361232
GACCGTA649450.016.00012869
TACGGTG32150.015.9884714
CGTACGT25250.015.57655634
GACGGCT53100.015.52867433
Produced by FastQC (version 0.11.2)