FastQCFastQC Report
Thu 13 Jul 2017
HVKFHBGX2_n01_ba87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVKFHBGX2_n01_ba87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40205699
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG73700.038.9048421
TTGACGG94950.029.2138331
TATGCCG36150.029.20231649
TGACGGC102600.027.13648832
CTACGGT52450.026.63904213
GACGGCT105050.025.97832333
TACGGTG53700.025.9545814
CCGTCTT36900.025.61710553
TGCCGTC41550.025.15801451
CTCGTAT38750.024.57209245
TCTCGTA38900.024.03411944
GACGCAC60950.023.7175256
CCTACGG60800.023.20769512
ATCTCGT41350.023.19417443
ACGCACC61450.023.074677
TCCGCAA60550.022.96262733
CTAGACG62450.022.7057723
CGGCTAT130650.022.3402735
ACGGTGA64200.021.87088215
CTTGACG129250.021.56810630
Produced by FastQC (version 0.11.2)