FastQCFastQC Report
Tue 1 May 2018
HVK5FBGX5_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVK5FBGX5_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437713829
Sequences flagged as poor quality0
Sequence length75
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG124292800.068.8205646
TGTCGGT124478700.068.76697
TCGGTCT124628850.068.728149
CGGTCTC124666050.068.6910810
ACGAGGT134367700.068.6351110
GTCGGTC124786550.068.6346748
GTCTCGT124712550.068.6077512
GGTCTCT134055750.068.5873914
CCACGAG134523950.068.5460368
CGCCGGT4216050.068.542321
CACGAGG134537150.068.537919
ACGCGTG2315850.068.536292
GGTCTCG125019550.068.48077411
TCCACGA134654700.068.467167
CGAGGTC134721100.068.4462111
TCACGTG2298500.068.4398042
GTCTCTG33904000.068.4313115
GAGGTCT134777950.068.4198212
TCGTAGC29601250.068.326215
GTCTCTA43190650.068.250215
Produced by FastQC (version 0.11.2)