FastQCFastQC Report
Tue 23 Jan 2024
HVK55DRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVK55DRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026971400
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCG34986873134.068011144224656Illumina Single End PCR Primer 1 (100% over 8bp)
TCTCAGTG196283501.911284968597957No Hit
CTGTTGGA188072311.8313295774351652No Hit
GGCTAACA180564381.758222088755344No Hit
GAGGCTAT179232731.7452553206447619No Hit
CACCTACA177447321.7278701237444392No Hit
GCTCACAA177428201.7276839452393709No Hit
TCAGGCAA174021291.6945096036754288No Hit
CCTGAACT173786841.692226677393353No Hit
GGTACCTT166313381.6194548358406085No Hit
TTCACGCA154798731.5073324339898853No Hit
TGTTACGG154588921.505289436492584No Hit
TTCCACGA152763621.4875158159224298No Hit
GTTCCTAG151466991.4748900504921558No Hit
GCATCCAT150873471.4691107269394261No Hit
GTCGGTAA144258251.4046958853966138No Hit
GGACGTAT142534211.387908270863239No Hit
TTCTCTGC135209291.3165828181777992No Hit
TAGCGACG135174561.3162446393346494No Hit
GGTCTATG133917541.3040045711107437No Hit
CCAATGCT133766091.3025298464981596No Hit
CTACACTA130128571.2671099701510675No Hit
AGCTCTAG129668251.2626276642173286No Hit
CACGTCGT128194211.2482743920619406No Hit
GTTAACCG123189511.1995417788655067No Hit
CGTACGCA117546181.1445905893776593No Hit
TGGTTCAC113295601.103201121277574No Hit
TAGCACAG111688651.087553655340353No Hit
GTGTATAT105765491.029877657741978No Hit
ACTAGAGC104234341.0149682844137626No Hit
GACCATTG90094410.8772825611307189No Hit
GAAGTCAG87655820.8535371092125836No Hit
GACTTCGA86980830.8469644821657155No Hit
TGCAACCT84912900.8268282836308781No Hit
TCAGTGTC84823410.8259568864332542No Hit
TTCCGTCT80751950.7863115759601484TruSeq Adapter, Index 14 (100% over 8bp)
CTCTAGCT78259150.7620382612407707No Hit
GGGGGGGG76493210.7448426509248456No Hit
TTATGGCC75630580.7364429038627561No Hit
TTCAGCAC73822290.7188349159480001No Hit
TCCAGAGA66153840.6441643847141215No Hit
GAAGGACT57037880.5553989137380066No Hit
TACTCCTT41374360.4028774316402579ABI Solid3 GAPDH Reverse Primer (100% over 8bp)
TCTACTCT33396550.3251945477741639No Hit
CTCCTTAC33337650.32462101670991034No Hit
AGAGGATA31802560.3096732781458179No Hit
GTTGTGAC28811640.2805495849251498No Hit
TATGCAGT22910640.22308936743515936No Hit
TCCTCAAG22642680.22048014190073842No Hit
AGATATCC22535600.2194374643733993No Hit
CACTCACG21799140.21226628122263191No Hit
ATAGAGAG21761270.21189752703921452ABI Solid3 Adapter B (100% over 8bp)
ACACGCAG21218690.20661422508942312No Hit
ACTCGCTA21075480.20521973640161745No Hit
GAGCTAAC20731720.20187241825819105No Hit
CGTACGAT20244730.19713041667956868RNA PCR Primer, Index 22 (100% over 8bp)
ACGGTGTC19410760.1890097426276915No Hit
TTGTGCGT19247880.1874237198815858No Hit
CTCGATGA18832420.1833782323441529No Hit
CAGATAGT18238450.17759452697514264No Hit
CCTATCAC17995490.17522873567852035No Hit
TCTCGAAC17581650.1711990226796968No Hit
GTTCGACA17043240.16595632556077025No Hit
CGAGAGTT16966800.16521200103527714TruSeq Adapter, Index 13 (100% over 8bp)
TCATTACA16630980.1619419976057756No Hit
GCGATCTA16582990.1614747012428973No Hit
AGGCTTAG16227280.15801102153380317No Hit
ACCTAGTA15305750.14903774340746No Hit
CGATCTCG14067100.13697655066148873Illumina Paired End PCR Primer 2 (100% over 8bp)
TATCTGAC12430210.12103754788108023No Hit
CTCGTCGA11750980.11442363438748147No Hit
CTGACGTG11581640.11277470823432864No Hit
CCATCTTA11421060.11121108143810042No Hit
GTCTCGTA11337680.11039917956819441TruSeq Adapter, Index 14 (100% over 8bp)
ACGCTATC11250750.10955271003652098No Hit
ATGTCAAT11149880.10857050157385102RNA PCR Primer, Index 15 (100% over 8bp)
AACCGTGA11116690.10824731827974957No Hit
ACACGATC11049990.1075978357333028No Hit
CGAGACGT10836290.10551695986860005No Hit
TGATCTCG10568040.10290491049702065Illumina PCR Primer Index 5 (100% over 8bp)
GTGACTCT10499740.10223984815935477No Hit
TCACGCGT10457840.10183185237680427No Hit
AGTGTAGA10385650.10112891167173692Illumina Single End PCR Primer 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)