FastQCFastQC Report
Tue 23 Jan 2024
HVK55DRX3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVK55DRX3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026971400
Sequences flagged as poor quality0
Sequence length8
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGG36323377535.369414863938765No Hit
AAGGACAC429814204.185259686881251No Hit
TTCACGCA420046154.0901445746200915No Hit
AGCACCTC322136383.136760965300494No Hit
TGGCTTCA310689983.025303138918961No Hit
ACAGATTC289020132.8142958022005287No Hit
AGCCATGC276228152.689735566151112No Hit
ATCATTCC206026482.006155965005452No Hit
TCTTCACA202711081.973872690125548No Hit
ACACGACC199573211.9433180904550995No Hit
AATCCGTC189899401.8491206278967456No Hit
TCCGTCTA165820121.6146517809551464No Hit
TATCAGCA140219461.3653686947854633No Hit
TGAGTACG122398081.1918353325126678No Hit
TAGTCTCC119514101.1637529535876072No Hit
ACTACGAC97088890.9453903974346315No Hit
CGAGCGAC93005990.9056336914543093No Hit
ACCGGTAA89637150.8728300515476868No Hit
GTCTGCTA85034970.8280169243272014No Hit
TCCTGAGC83945990.8174131236760829No Hit
ACTCACTG83845260.8164322784451447No Hit
ACGCTACT79120750.7704279788122629No Hit
GAACCACA76968130.7494671224534588No Hit
CTGCGTAG76190590.7418959281631406No Hit
GACATAGT74121590.7217493106429254No Hit
CGAGAGTT72518870.7061430337787401TruSeq Adapter, Index 13 (100% over 8bp)
GGTTGCAA69482720.6765789193350467No Hit
CATTACGG69260160.6744117703764682No Hit
AGGCAGAA65520640.6379986823391576No Hit
AACCTCCA65114170.6340407337536371No Hit
TAGGCATG64442970.6275050113372194No Hit
GTAGAGGA63850150.6217325039431478No Hit
AAGAGGCA63503670.6183587001546489No Hit
ACAGTGGT63182320.6152295964619853No Hit
CACTAGCT62727310.6107989959603549Illumina PCR Primer Index 10 (100% over 8bp)
CGTGTACA61623980.60005546405674No Hit
TAAGGCGA58657200.5711668309360903No Hit
CATCTTCG57846850.5632761535520853No Hit
GGACTCCT57840890.5632181188297941No Hit
GCATAAGG57355150.5584882889630617No Hit
GCTACGCT56638720.5515121453236185No Hit
GCGACTAA56399960.5491872509789464No Hit
CGAGGCTG56184460.5470888478491222No Hit
CAGAGAGG55747210.542831183030024No Hit
CTCTCTAC54919420.5347706859217306No Hit
GTCTATGA54584670.5315111014776068No Hit
GATTCGGA53667570.5225809598982016No Hit
CGTACTAG40944310.3986898758816458No Hit
ACTCGCTA20675360.20132362011249777No Hit
GCGTAGTA20641150.20099050470149413No Hit
GCTCATGA20113350.19585112107308927No Hit
ATAGTACC18095840.17620588070904408No Hit
ATCTCAGG17329960.16874822414723525No Hit
CGAAGTAT16730510.16291115799329953No Hit
ACGTGCGC16346900.15917580567482212No Hit
TAGCAGCT15693100.15280951348791214No Hit
ACTGAGCG15327850.14925293927367403No Hit
CGGAGCCT15247100.14846664668558443No Hit
GGAGCTAC14883000.14492127044628508No Hit
TATAGCGA14455640.14075990821166No Hit
CTCGACTT13549490.1319363908284106No Hit
TCTCTATG13223180.12875898978296768ABI Solid3 Adapter B (100% over 8bp)
TGCTCGTA13040710.12698221196812295No Hit
CCTAAGAC12799070.12462927399925645No Hit
AACGCTGA11806870.11496785596950411No Hit
TAGCGCTC11604030.11299272793770108No Hit
TACGCTGC11521620.11219027131622165No Hit
ATGCGCAG11439840.11139394923753475No Hit
GCGTATAC10956250.10668505471525301No Hit
CTACGACC10700550.10419520933104855No Hit
AGTCGCAG10618300.10339431068869104No Hit
CGATCAGT10414910.10141382710365644No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)