FastQCFastQC Report
Tue 23 Jan 2024
HVK55DRX3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVK55DRX3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026971400
Sequences flagged as poor quality0
Sequence length301
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG34583917133.67563799731911No Hit
GTAACCGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACATCT394613903.8425013588499155No Hit
GTAACAGGATTAGATACCCGGTTCAGAAGGACTGTCTCTTATACACATCT311989853.0379604534264537No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTG171793811.67281980783496No Hit
GGCTTGGCATCGATGAAGAACGCAGCCGCTGTCTCTTATACACATCTCCG54025260.5260639195989294No Hit
GGCTTGGTCATTTAGAGGAAGGAGAAGTCGTAACAAGGTTTCCGTAGGTG46244760.45030231611123733No Hit
GTAACCGGATTAGAAGGACTGTCTCTTATACACATCTCCGAGCCCACGAG36724400.3575990529044918No Hit
GTAACCGGTTCAGAAGGACTGTCTCTTATACACATCTCCGAGCCCACGAG29439490.2866631923732248No Hit
CTGTAAAGATAAATGGGTCATCTAATGAGTATGGATCTGGGATTGATCCG27703830.26976242960612146No Hit
CTGTAAAGATAAATGGGTCATCTAATCCTGTTACTCCTGTCTCTTATACA24244700.23607960260626537No Hit
CTGTAAAGATAAATGGGTCATCTAATCCGGTTACCTGTCTCTTATACACA23966510.23337076378173727No Hit
GGCTTGGTCATTTAGAGGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTG22544160.21952081625642156No Hit
GTAACCGGATTAGATACCCTGTTCAGAAGGACTGTCTCTTATACACATCT21512210.20947233778856938No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAAC19450270.18939446609710844No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCGTCTGTCTCTTATACACATC19298470.18791633340519512No Hit
CTGTAAAGATAAATGGGTCATCTAATCCTGTTACCTGTCTCTTATACACA19162690.18659419337286315No Hit
CTGTAAAGATAAATGGGTCATCTAATCCGGTTACTCCTGTCTCTTATACA17910110.17439735906959045No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATCGACTCG16575180.16139865238700904No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCTAC15987210.15567337123507044No Hit
GTAACAGGATTAGATACCCTGTTCAGAAGGACTGTCTCTTATACACATCT15786750.15372141814270585No Hit
GTAACAGGATTAGATACACCGGTTCAGAAGGACTGTCTCTTATACACATC15300270.14898438262253458No Hit
CTGTAAGGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG15266870.1486591544808356No Hit
GTAACCGGATTAGATACACCGGTTCAGAAGGACTGTCTCTTATACACATC14927820.14535769934781048No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGGCTCG14520040.14138699480822933No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAAC14386910.14009065880510402No Hit
CTGTAAAGATAAATGGGTCATCTAAGGAGTATGGAGCTGGGATTGACTCG13707970.1334795691486637No Hit
CTGTAAAGATAAATGGGTCATCTAAAGGGTATGGAGCTGGGATTGACTCG13551010.13195119163006877No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAAC13026400.12684287021040702No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGGGCTGGGATTGACTCG12320100.11996536612411991No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATCGACTCG12080200.11762937117820418No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGGTTGACTCG11172280.1087886186509186No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG10815240.10531198824037359No Hit
GAGTAACAGGATTAGATACCCGGGTAGTCCACGCCGTAAACGATGATAAC10460130.10185415095298662No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCGTCTGTCTCTTATACACATC10451250.10176768311172053No Hit
CTGTAAAGATAAATGGGTATCTAATCCTGTTACTCCTGTCTCTTATACAC10430600.10156660643129886No Hit
GAGTAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCAAC10419210.1014556977925578No Hit
GAGTAACCGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAAC10402340.10129142836889128No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCCGGGATTGACTCG10302440.10031866515464792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)