FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n02_atac_mp-1000_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_atac_mp-1000_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38420177
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7204591.8752099970804403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA118000.027.21291548
CATTAAA121500.026.66179853
ATCATTA129050.025.15528751
AGGGGGG121850.023.91725366
ATTAAAA139800.023.4221554
GTATCAT139400.023.23640449
AAGGGGG128450.022.6052665
AAAGGGG130600.022.44753364
TCATTAA145900.022.29906752
TATCATT147850.022.19277850
GCCGTAT157200.020.73647746
TTAAAAA164400.020.04521255
CCGTATC164800.019.97366747
AAAAGGG156350.018.9294963
TAAAAAA184000.018.06215356
GTGTAGA1835350.018.02743570
TGTAGAT1395050.017.22854470
AGTGTAG2379700.017.0171470
CGCCGTA196900.016.55209245
GAGTGTA3022900.015.80555370
Produced by FastQC (version 0.11.2)