FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n02_atac_mp-1000_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_atac_mp-1000_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52514010
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1160870.22105910403718929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATA33050.022.2592970
GTGTAGA2371650.017.93507270
TGTAGAT1788350.016.88566870
AGTGTAG3028800.016.80808670
ATTAAAA28450.016.00315354
AGTGTAT16900.015.753983570
CATTAAA30100.014.89338253
GAGTGTA3800000.014.81295570
CCTTATA13950.014.2920072
ACACATA81000.014.27224970
GTAGATC1405900.014.08850470
TTAAAAA32900.013.94501855
TAGATCT1034250.013.59613170
TCGGTGG511700.013.32950570
CGAGTGT4599600.012.2209169
AGATCTC936400.011.79202270
ATCATTA40300.011.29720951
TCTCGGT756400.011.12907669
TCATTAA41500.011.0554652
GTGTAAG19950.011.0444431
Produced by FastQC (version 0.11.2)