FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n02_9_mp-25hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_9_mp-25hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43647777
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1319200.30223761452960135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA91900.023.43489648
GTATCAT111050.019.4568349
ATCATTA111650.019.3216851
TCATTAA114650.019.00011452
AGTGTAG1600900.017.85855170
GTGTAGA1242450.017.8029870
CATTAAA126450.017.19941553
AGGGGGG112850.017.00991666
TATCATT130500.016.95951850
TGTAGAT929050.016.57622570
CCGTATC132700.016.20298247
GAGTGTA2035950.015.70808370
GCCGTAT141850.015.35354446
AAGGGGG132550.014.40180865
ATTAAAA161400.013.8220654
GTAGATC740600.013.79811670
AAAGGGG139350.013.72402264
TAGATCT558900.013.15665270
CGAGTGT2504600.013.14091170
GTCCTAC130500.012.3551091
Produced by FastQC (version 0.11.2)