FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n02_8_mp-25hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_8_mp-25hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40446539
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1538200.38030448044021764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG1336100.018.88025970
GTGTAGA1021900.018.35714570
TGTAGAT758850.017.23742570
GAGTGTA1747950.017.03306870
CGAGTGT2207050.013.91848470
TAGATCT458200.013.71580970
GTAGATC615200.013.19930870
GTCCTAA42500.012.3437071
ACGAGTG2615650.011.68123869
AGATCTC442350.011.43550170
TATACCG42200.011.0253865
GTCCTAC67500.010.6216831
TAGGACG57500.010.281524
TCTATAC40350.010.1427583
CATACAC152150.010.1386553
GTATTAG23450.09.9925241
TACACCG93300.09.9361665
GACGAGT3102150.09.8844568
TCATACA156150.09.6094942
GTGTATG139900.09.5246831
Produced by FastQC (version 0.11.2)