FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n02_6_mp-25hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_6_mp-25hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38367133
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3985911.0388865907702824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG93450.022.48893766
AAGGGGG99850.020.90634765
CGTATCA112200.020.87980548
AAAGGGG119850.017.47592764
AGTGTAG1172700.017.06568170
CCGTATC138800.016.8529847
GCCGTAT140550.016.74124746
GTGTAGA932750.016.2918670
GTATCAT143850.016.13979149
TCATTAA152100.015.95645152
CATTAAA154350.015.79171253
GAGTGTA1483900.015.3658370
ATCATTA158850.015.03509551
TGTAGAT733300.014.43640870
CTAGTAC43200.014.0080593
CGAGTGT1838150.013.178246570
TATCATT182000.013.0452950
AAAAGGG163300.012.97614863
CGCCGTA183550.012.94997545
GTAGATC597550.011.36124470
Produced by FastQC (version 0.11.2)