FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n01_atac_mp-1000_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_atac_mp-1000_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38420177
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT5421381.4110762685970968No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3268120.8506259614576998No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG781990.20353628251113992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG623000.062.1891970
AAGGGGG724650.053.43796569
AAAGGGG741950.052.2769968
CGTATGC796650.050.2018441
GTATGCC796850.050.1339742
AAAAGGG778100.049.9668367
TCGTATG805400.049.7154640
CTCGTAT809750.048.78623239
TGAAAAA849000.047.32203759
TATGCCG849850.047.0458943
TCTCGTA840800.046.85324538
GAAAAAA863950.046.55155660
AAAAAGG840500.046.4477366
CCGTCTT865300.046.11024547
AAAAAAG854600.045.90718565
TTGAAAA882500.045.40255758
CTTGAAA886050.045.17352757
GCCGTCT894250.044.65611346
TCTGCTT906050.044.11364753
TTCTGCT910350.043.89678652
Produced by FastQC (version 0.11.2)