FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n01_atac_mp-1000_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_atac_mp-1000_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52514010
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT585070.11141217362757101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG109900.037.83639570
TCGTATG119300.035.76045240
AAAAAAG118500.035.54184765
AAAGGGG119700.034.94805568
GAAAAAA125550.034.6094460
AAAAGGG122800.034.1527467
TGAAAAA138150.031.1995259
CGTATGC138700.031.13788841
AAGGGGG134200.031.06437969
CTCGTAT139100.030.59408439
GTATGCC142250.030.21448342
TTGAAAA142750.030.04685858
AAAAAGG144150.029.07143466
CTTGAAA191200.022.72615257
TGTATAA14350.020.72573714
GTGTATA16150.020.58244313
TCTCGTA213800.020.00217238
CCGTCTT230850.018.77102347
TCTGCTT240750.017.8855853
CAGGCAG2574650.016.45922370
Produced by FastQC (version 0.11.2)