FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n01_9_mp-25hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_9_mp-25hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43647777
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT789350.18084540708682598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG146350.038.40951570
TCGTATG165300.035.03982540
CGTATGC176350.032.94464541
CTCGTAT179150.031.91990339
GTATGCC192500.030.18168442
AAGGGGG197100.028.50262669
AAAGGGG210100.026.79225368
TCTCGTA213700.026.77451738
AAAAGGG223250.025.21533467
CCGTCTT245950.023.59569247
TATGCCG266300.021.9627343
GCCGTCT289100.020.146346
AAAAAAG297950.019.2354965
AAAAAGG303350.018.75428266
GAAAAAA318600.018.7157760
TGCTTGA337550.017.66511255
GCTTGAA338400.017.41396156
ACGCTAC1662000.017.30065770
TTGAAAA347100.016.9472458
CTTGAAA348250.016.93157257
Produced by FastQC (version 0.11.2)