FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n01_8_mp-25hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_8_mp-25hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40446539
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2475290.6119905586977418No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT765150.18917564244495677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG121850.045.7306270
TCGTATG139750.040.7948840
CGTATGC149350.038.9002341
GTATGCC155050.037.53964242
CTCGTAT151850.036.92149439
AAGGGGG152050.036.64869769
AAAGGGG156900.035.7652668
AAAAGGG183600.030.54624267
TCTCGTA189300.029.72654538
CCGTCTT226550.025.66233647
AAAAAGG221800.025.52342866
TATGCCG245450.024.07079143
AAAAAAG251050.022.95443365
GAAAAAA266250.022.68551360
TTGAAAA262900.022.4418158
CTTGAAA267050.022.01460857
GCCGTCT274150.021.25753446
TGAAAAA282600.021.15005759
TCTGCTT283550.020.47038753
TGCTTGA299750.019.50742355
Produced by FastQC (version 0.11.2)