FastQCFastQC Report
Tue 19 Jun 2018
HVJJYBGX5_n01_6_mp-25hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_6_mp-25hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38367133
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT2966030.773065321299874No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2653160.6915189623368522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG345150.061.07048870
AAGGGGG399550.052.7397469
TCGTATG439800.049.57642740
CGTATGC442150.049.40933241
GTATGCC445350.049.0249942
AAAGGGG431950.048.89418868
CTCGTAT443100.048.21093839
AAAAGGG479200.044.24265367
TCTCGTA481500.044.14555438
CCGTCTT502650.043.4455747
TATGCCG507750.043.16678643
GCCGTCT524500.041.56835646
AAAAAGG548700.038.78133466
TCTGCTT582300.037.65453
TGCTTGA583100.037.62072455
TTCTGCT591800.037.0726352
GCTTGAA594400.036.88881356
CTTGAAA602450.036.4658457
TCTTCTG616600.035.65875650
CTGCTTG618550.035.48634354
Produced by FastQC (version 0.11.2)