FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah9251-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah9251-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5608306
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC210630.37556795224796935TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG35650.029.53060272-73
GATCTCG38050.027.73095338-39
TGCCGTC39400.027.6742348-49
AAGGGGG37700.027.61316372-73
GCCGTCT39500.027.12823548-49
GTATGCC42050.025.42723744-45
CGATCAG42000.025.40212232-33
TATGCCG42950.025.2774146-47
CTCGTAT45100.024.33313242-43
CGTATGC45400.024.27564244-45
AGTCACG46850.023.22419528-29
ATGCCGT46700.023.09668246-47
GTCACGA46900.022.69836828-29
TCTCGTA47250.022.67883340-41
CACGATC47750.022.3431330-31
TCGTATG49350.021.95186442-43
AAAGGGG47850.021.80493470-71
ATCTCGT49300.021.73579640-41
GAGCACA99400.021.6552339
CCGTCTT51550.021.3795250-51
Produced by FastQC (version 0.11.2)