FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah9251-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah9251-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8330153
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC796740.9564530207308318TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG104350.038.89106472-73
AAGGGGG112150.037.2129172-73
GCCGTCT107100.037.10257748-49
TATGCCG116350.035.68782846-47
TGCCGTC113400.035.60082648-49
GTATGCC118450.035.41221644-45
CGTATGC118150.035.36290744-45
ATGCCGT118950.034.43363646-47
CATCTCG117400.034.2700138-39
AAAGGGG120000.033.97568570-71
TCGTATG123350.033.16845342-43
CTCGTAT122850.033.01651842-43
CCGTCTT121150.032.7415350-51
ATCTCGT128100.031.48011840-41
TCTCGTA129150.031.27876940-41
CTGCTTG134200.029.33001356-57
GAGCACA308800.028.2931969
CAGATCA156750.027.31614934-35
GCTTGAA147100.027.12532658-59
AGAGCAC325300.026.8725478
Produced by FastQC (version 0.11.2)