FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah9120spike-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah9120spike-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5502875
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT371020.6742293800967676TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG59600.036.39217874-75
GCCGTCT49800.034.8712750-51
AAGGGGG63550.033.6125174-75
TGCCGTC54800.033.53349350-51
GTATGCC58300.032.40709746-47
ATGCCGT58800.031.4521348-49
TATGCCG59450.031.06871448-49
GTTTCGG72950.030.82868834-35
AAAGGGG70600.030.7220172-73
CGTATGC61050.030.67787646-47
CGTTTCG72900.030.6558632-33
CTCGTAT59150.030.39200444-45
ATCTCGT64500.028.89149942-43
CGGAATC78800.028.30143238-39
AGTCACG80200.028.21761728-29
TCGTATG65050.028.17733444-45
TCTCGTA64650.028.17018942-43
GTCACGT81800.027.60822728-29
CCGTCTT63600.027.34180852-53
TCGGAAT85250.026.60120636-37
Produced by FastQC (version 0.11.2)