FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah9120spike-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah9120spike-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7999313
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT6638238.298500133698981TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT264090.3301408508455663TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT167830.20980551704877656TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGAT103580.12948611962052242TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTTT96570.12072286707621017TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1052250.074.1433949
AGAGCAC1071400.072.8839658
AAGAGCA1129950.069.277917
GAAGAGC1131050.069.1457446
CGGAAGA1136700.068.868234
TCGGAAG1144150.068.456793
GGAAGAG1163300.067.38245
ATCGGAA1168200.067.092132
GATCGGA1163600.066.903321
TGCCGTC750350.045.07864850-51
GCCGTCT705400.045.0462650-51
AGGGGGG836050.044.9381574-75
CGTATGC763550.044.8594746-47
GTATGCC763150.044.84603546-47
CTCGTAT753200.044.8082444-45
TATGCCG762650.044.7460248-49
ATGCCGT752200.044.7303548-49
AAAGGGG841150.044.5930572-73
CCGTCTT722950.044.49231752-53
ATCTCGT772050.044.47655542-43
Produced by FastQC (version 0.11.2)