FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah9120-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah9120-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5554514
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC183310.3300198721256261TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG32000.028.27234372-73
TGCCGTC33850.027.21312348-49
GCCGTCT33750.026.59748648-49
AAGGGGG35900.025.2664372-73
CTTAGGC37550.024.4696432-33
ACTTAGG38250.024.32901632-33
GTATGCC37850.024.15097844-45
CATCTCG37400.023.7512738-39
CGTATGC39900.023.32240544-45
TATGCCG40650.022.77648446-47
GAGCACA82750.022.2637889
GCATCTC40400.022.1620738-39
CTCGTAT41200.022.13100642-43
ATGCCGT42200.021.71716746-47
AGAGCAC86150.021.6033388
TTAGGCA44050.021.44602434-35
TCTCGTA41700.021.35845440-41
TAGGCAT42400.021.28295934-35
CCGTCTT43700.020.8641850-51
ATCTCGT43200.020.72563740-41
Produced by FastQC (version 0.11.2)