FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah9120-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah9120-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6630414
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC960681.4488989676964363TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA207000.048.0202269
AGAGCAC218100.045.6840258
AGGGGGG116800.041.36513572-73
TCGGAAG248800.040.137363
CGGAAGA249500.040.0812844
TGCCGTC125050.039.6696848-49
GCCGTCT124450.039.48328848-49
AAGGGGG122850.039.30889572-73
CGTATGC128600.038.6842444-45
GTATGCC129100.038.51621644-45
ATGCCGT128750.038.3106446-47
TATCTCG129450.038.21267738-39
GAAGAGC261950.038.1942446
TATGCCG130900.038.0763446-47
AAAGGGG126900.038.05435670-71
GTCACGC131100.037.83900528-29
GATCGGA263100.037.7905161
TCACGCC131800.037.74501430-31
AAGAGCA265750.037.7049267
ATCGGAA266750.037.595942
Produced by FastQC (version 0.11.2)