FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8584spike-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8584spike-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6700255
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC199320.29748121526718013TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG35850.027.8582872-73
AAGGGGG40900.024.41856672-73
GCCGTCT41550.024.26279448-49
CATCTCG42600.023.61048738-39
TGCCGTC44100.023.07298948-49
GTATGCC46350.021.85154244-45
CACGGCT46500.021.6298530-31
CGTATGC48150.021.32748644-45
TCACGGC47200.021.30906530-31
CGGCTAC47400.021.26873232-33
TATGCCG49300.020.68699346-47
GTCACGG48850.020.63780428-29
ATGCCGT49650.020.54116246-47
CTCGTAT50500.020.28894242-43
ACGGCTA50850.020.10300432-33
GGCTACA51650.019.65550434-35
CCGTCTT52850.019.47527950-51
GAGCACA106100.019.4461529
AGTCACG52250.019.38481928-29
ATCTCGT52700.019.3527840-41
Produced by FastQC (version 0.11.2)