FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8584spike-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8584spike-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8456199
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT3312993.917824072021011TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA538850.062.4755949
AGAGCAC554750.060.7866138
GAAGAGC608650.055.5568356
TCGGAAG610750.055.4277233
CGGAAGA612350.055.351674
AAGAGCA616850.054.8803947
GATCGGA624250.053.922921
ATCGGAA635150.053.447092
GGAAGAG636150.053.2808275
AGGGGGG370400.044.45437274-75
GCCGTCT383300.043.5590750-51
TGCCGTC386650.043.44301250-51
CGTATGC392800.042.86453246-47
GTATGCC391600.042.7918546-47
TATGCCG393250.042.77364748-49
AAAGGGG384350.042.77364772-73
ATGCCGT394150.042.50904548-49
CTCGTAT395050.042.39435644-45
CCGTCTT398950.042.0623952-53
ATCTCGT398850.041.85543442-43
Produced by FastQC (version 0.11.2)