FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8584-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8584-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7137077
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT3414744.784507719336641TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA627150.055.9120529
AGAGCAC646400.054.3560338
GAAGAGC702200.050.176116
AAGAGCA704000.050.042247
CGGAAGA717250.049.097054
TCGGAAG722550.048.7173923
GGAAGAG726150.048.4240725
GATCGGA742350.047.1351971
ATCGGAA748800.047.1106722
AGGGGGG383800.044.47548374-75
TGCCGTC399100.043.9539550-51
GCCGTCT397450.043.94130750-51
GTATGCC403500.043.340746-47
CGATCTC393900.043.30141440-41
TATGCCG405650.043.2731948-49
GATCTCG395400.043.23224340-41
CGTATGC406950.043.11763446-47
ATGCCGT406700.042.99968748-49
CTCGTAT405750.042.92082644-45
AAAGGGG398800.042.79673872-73
Produced by FastQC (version 0.11.2)