FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8584-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8584-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7510212
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC5813307.7405271648789675TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA781750.077.072559
AGAGCAC791100.076.238868
GAAGAGC840050.071.975246
CGGAAGA843750.071.6930544
TCGGAAG846650.071.470183
AAGAGCA847250.071.330497
GATCGGA854000.070.419221
GGAAGAG859950.070.402625
ATCGGAA865050.070.054642
GCCGTCT632500.045.2481448-49
GTATGCC653100.045.0118944-45
TGCCGTC642000.044.89336448-49
CGTATGC652600.044.81952344-45
AAGGGGG644650.044.81088372-73
TATGCCG653250.044.7317646-47
ATGCCGT649000.044.7169246-47
AGGGGGG643700.044.70543772-73
TATCTCG653100.044.62656438-39
TCGTATG654700.044.49089442-43
CTCGTAT652100.044.41962442-43
Produced by FastQC (version 0.11.2)